The Australian Ecosystems Foundation Inc. currently owns, manages or assists in the management of thousands of hectares of land for wildlife conservation.  These reserves have been identified as being of high conservation value due to factors such as their location, the number of threatened species of flora and fauna present, and potential connectivity with larger regional protected areas.

AEFI works with landholders to increase the biodiversity of their ecosystems and therefore improve the conservation value of their land.  Through these partnerships, AEFI has been able to provide safe habitats for several threatened species including Spotted-tailed Quoll, Regent Honeyeater, Purple Copper-wing Butterfly as well as many less threatened native species. Within its own land, AEFI has established both captive and wild breeding programs for species such as the Eastern and Spotted-tailed Quoll, Rufous Bettong, Long-nosed Potoroo, Brush-tailed Rock Wallaby and Red-necked Pademelon.  These species are being bred to increase their numbers to help secure their future, and for eventual reintroduction into protected habitats.